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KMID : 0644120140270020059
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Journal of Oriental Gynecology
2014 Volume.27 No. 2 p.59 ~ p.70
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Inhibitory Effects of Ursolic Acid from Prunella Vulgaris on Osteoclast Differentiation
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Heo Ja-Kyung
Hwang Deok-Sang
Lee Jin-Moo
Lee Chang-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Purpose: This study was conducted to evaluate the inhibitory effect of ursolic acid from Prunella vulgaris on osteoclast differentiation.
Methods: MTT-assay was performed to estimate cytotoxicity of ursolic acid from Prunella vulgaris in BMMs stimulated with M-CSF. TRAP staining, TRAP activity and Real-time PCR were performed to know the inhibitory effect on osteoclast differentiation. Actin ring formation were analysed to observe the effect of ursolic acid from Prunella vulgaris.
Results: Ursolic acid from Prunella vulgaris has no cytotoxicity at the concentration of 1 μg/ml or lower. Ursolic acid decreased the number of TRAP positive cells and the expression of NFATc1 gene, c-Fos gene, TRAP and OSCAR in BMMs stimulated with RANKL. Ursolic acid restrained the formation of actin ring. Ursolic acid inhibited NF-κB activity by inducing degradation of p-IkB?.
Conclusions: Ursolic acid from Prunella vulgaris has the inhibitory effect of osteoclast differentiation and bone resorption. Futher studies are needed to treat osteoporosis by usolic acid from Prunella vulgaris.
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KEYWORD
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Ursolic Acid, Prunella Vulgaris, Osteoclast, NFATc1, C-Fos, OSCAR, TRAP
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